Ultimate Spider Slayer

Alistair helped his father, Spencer Smythe, in his lab until an explosion occurred. Alistair was subsequently paralyzed as of the accident and Spencer blamed himself. Spencer, a master of robotics, was hired by Norman Osborn to create "Spider Slayer" robots to capture Spider-Man. In exchange, Norman offered funds to build a hoverchair for Alistair. Spencer stayed behind at Oscorp to fight Spider-Man whilst Norman took Alistair to safety, however, the building caught fire, and as it exploded, Spencer was believed dead, though no body was recovered. Alistair then began to work for the Kingpin, who falsely explained to him that Spider-Man was responsible for his father's demise and also supplied him with a hoverchair. Alistair soon took on updating his fathers Spider Slayer inventions, designing a number of more advanced robots with the intention to kill Spider-Man. However, Alistair was unsuccessful in destroying the wall-crawler.

Smythe later devised a plan that failed causing his employers son Richard being imprisoned. Kingpin, feeling that Alistair had failed him too often, dismissed him. Herbert Landon then took Alistair's place, and he and Kingpin turned Alistair into a cyborg against his will. However, Smythe's programming wasn't strong enough and, having resisted it, began to attack Kingpin having discovered that he was keeping his father, who apparently survived the explosion, under cryogenic suspension.

Alistair later took on the role as Silvermane's top scientist, in exchange for bringing his father out of cryogenic suspension. Alistair, using his father's knowledge of robotics, began creating devices for the Green Goblin to use in crashing Peter Parker and Mary Jane Watson's wedding. Alistair also appeared as Red Skull’s employee during the Secret Wars.

In Ultima, the Ultimate Spider Slayer is a member of the Organization, having been recruited by Padro Lodo because of his genius intellect and ability to create Spider Slayer drones. Because of this, Smythe is currently a member of the Org Tech Division under Eggman's supervision, creating Spider Slayers and Genetic Mutations for the Organization's Armies.